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Closes #9673. Adds hisat3n/align for nucleotide conversion read alignment.

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the module conventions in the contribution docs
  • If necessary, include test data in your PR.
  • Remove all TODO statements.
  • Broadcast software version numbers to topic: versions - See version_topics
  • Follow the naming conventions.
  • Follow the parameters requirements.
  • Follow the input/output options guidelines.
  • Add a resource label
  • Use BioConda and BioContainers if possible to fulfill software requirements.
  • Ensure that the test works with either Docker / Singularity. Conda CI tests can be quite flaky:
    • For modules:
      • nf-core modules test <MODULE> --profile docker
      • nf-core modules test <MODULE> --profile singularity
      • nf-core modules test <MODULE> --profile conda
    • For subworkflows:
      • nf-core subworkflows test <SUBWORKFLOW> --profile docker
      • nf-core subworkflows test <SUBWORKFLOW> --profile singularity
      • nf-core subworkflows test <SUBWORKFLOW> --profile conda

then {
assertAll(
{ assert process.success },
{ assert snapshot(process.out.summary).match("se_summary") },
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according to the nf-core guidline all output need to be captured and the sam output seems to be missing here (https://nvnieuwk.github.io/nft-bam/latest/ might be helpful if it is too unstable)

channels:
- conda-forge
- bioconda
- nodefaults
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I don't think this is needed

Suggested change
- nodefaults

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Here is some additional context for nodefaults:
https://blog.fulcrumgenomics.com/p/anaconda-licensing-changes and #8847

It is not strictly necessary but may be a nice to have

@emmcauley emmcauley requested a review from mashehu January 21, 2026 13:21
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new module: hisat3n/align

2 participants