diff --git a/.changeset/tooltip-reduced-motion.md b/.changeset/tooltip-reduced-motion.md
new file mode 100644
index 00000000000..16316b2ffd8
--- /dev/null
+++ b/.changeset/tooltip-reduced-motion.md
@@ -0,0 +1,5 @@
+---
+'@primer/react': patch
+---
+
+Tooltip: Respect prefers-reduced-motion for the bubble fade-in.
diff --git a/e2e/components/Tooltip.test.ts b/e2e/components/Tooltip.test.ts
index 180e4980fb2..1275a736dcf 100644
--- a/e2e/components/Tooltip.test.ts
+++ b/e2e/components/Tooltip.test.ts
@@ -1,4 +1,4 @@
-import {test, expect} from '@playwright/test'
+import {test, expect, type Page} from '@playwright/test'
 import {visit} from '../test-helpers/storybook'
 import {themes} from '../test-helpers/themes'
 
@@ -21,4 +21,56 @@ test.describe('Tooltip', () => {
       })
     }
   })
+
+  // The bubble is drawn by the trigger's `::after`, which `toHaveCSS` cannot
+  // target, so these read the computed style of the pseudo-element directly.
+  const describeBubble = (page: Page) =>
+    page.locator('[role="tooltip"]').evaluate(element => {
+      const {animationName, display, opacity} = getComputedStyle(element, '::after')
+      return {animationName, display, opacity}
+    })
+
+  test.describe('reduced motion', () => {
+    // `contextOptions` is what the root Playwright config uses to emulate
+    // `prefers-reduced-motion`, so it is the option that has to be overridden.
+    test.use({contextOptions: {reducedMotion: 'reduce'}})
+
+    test('bubble is shown without the fade @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'deprecated-components-tooltip--default',
+      })
+
+      await page.locator('[role="tooltip"]').hover()
+
+      // `tooltip-appear` is what lifts the bubble off the `opacity: 0` in
+      // `.Tooltip::after`, so this is the case the visible-by-default rule
+      // exists for - without it, reduced motion would render nothing at all
+      expect(await describeBubble(page)).toEqual({
+        animationName: 'none',
+        // The rule asks for `inline-block`, but the bubble is absolutely
+        // positioned, so the computed value is blockified to `block`
+        display: 'block',
+        opacity: '1',
+      })
+    })
+  })
+
+  test.describe('no preference for reduced motion', () => {
+    test.use({contextOptions: {reducedMotion: 'no-preference'}})
+
+    test('bubble keeps its fade @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'deprecated-components-tooltip--default',
+      })
+
+      await page.locator('[role="tooltip"]').hover()
+
+      // Names are scoped by CSS Modules, so only the base name is stable
+      const bubble = await describeBubble(page)
+      expect(bubble.animationName).toMatch(/tooltip-appear/)
+
+      // The fade still lands on a fully opaque bubble
+      await expect.poll(async () => (await describeBubble(page)).opacity, {timeout: 1000}).toBe('1')
+    })
+  })
 })
diff --git a/packages/react/src/Tooltip/Tooltip.module.css b/packages/react/src/Tooltip/Tooltip.module.css
index 00b804da962..1bb480686f9 100644
--- a/packages/react/src/Tooltip/Tooltip.module.css
+++ b/packages/react/src/Tooltip/Tooltip.module.css
@@ -45,11 +45,20 @@
 .Tooltip:focus-within::after {
   display: inline-block;
   text-decoration: none;
-  animation-name: tooltip-appear;
-  animation-duration: 0.1s;
-  animation-fill-mode: forwards;
-  animation-timing-function: ease-in;
-  animation-delay: 0s;
+  /* `tooltip-appear` only fades the bubble in, so the visible state is the
+     default here and the fade is the enhancement. Without this the
+     reduced-motion path would inherit `opacity: 0` from `.Tooltip::after` and
+     render nothing at all. */
+  opacity: 1;
+
+  /* Only show animations if users don't have a preference for reduced motion */
+  @media screen and (prefers-reduced-motion: no-preference) {
+    animation-name: tooltip-appear;
+    animation-duration: 0.1s;
+    animation-fill-mode: forwards;
+    animation-timing-function: ease-in;
+    animation-delay: 0s;
+  }
 }
 
 .Tooltip--noDelay:hover::after,