diff --git a/.changeset/avatarstack-reduced-motion.md b/.changeset/avatarstack-reduced-motion.md
new file mode 100644
index 00000000000..acd77fd126b
--- /dev/null
+++ b/.changeset/avatarstack-reduced-motion.md
@@ -0,0 +1,5 @@
+---
+'@primer/react': patch
+---
+
+AvatarStack: Respect prefers-reduced-motion for the hover expansion.
diff --git a/e2e/components/AvatarStack.test.ts b/e2e/components/AvatarStack.test.ts
index 273f4946c98..09e20bc67ca 100644
--- a/e2e/components/AvatarStack.test.ts
+++ b/e2e/components/AvatarStack.test.ts
@@ -1,4 +1,4 @@
-import {test, expect} from '@playwright/test'
+import {test, expect, type Page} from '@playwright/test'
 import {visit} from '../test-helpers/storybook'
 import {themes} from '../test-helpers/themes'
 
@@ -84,4 +84,55 @@ test.describe('AvatarStack', () => {
       }
     })
   }
+
+  const lastItem = (page: Page) => page.locator('.pc-AvatarItem').last()
+
+  const describeTransition = (page: Page) =>
+    lastItem(page).evaluate(element => {
+      const {transitionDuration, transitionProperty} = getComputedStyle(element)
+      return {transitionDuration, transitionProperty}
+    })
+
+  test.describe('reduced motion', () => {
+    // `contextOptions` is what the root Playwright config uses to emulate
+    // `prefers-reduced-motion`, so it is the option that has to be overridden.
+    test.use({contextOptions: {reducedMotion: 'reduce'}})
+
+    test('still expands on hover, without sliding the avatars @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'components-avatarstack-features--align-left',
+      })
+
+      expect(await describeTransition(page)).toEqual({
+        transitionDuration: '0s',
+        transitionProperty: 'all',
+      })
+
+      const body = page.locator('[data-component="AvatarStack.Body"]')
+      const collapsed = await lastItem(page).boundingBox()
+      await body.hover()
+
+      // The reveal itself is unchanged - the last avatar simply reaches its
+      // expanded position in the same frame instead of over 200ms
+      const expanded = await lastItem(page).boundingBox()
+      expect(expanded).not.toBeNull()
+      expect(collapsed).not.toBeNull()
+      expect(expanded!.x).toBeGreaterThan(collapsed!.x)
+      await expect(lastItem(page)).toBeVisible()
+    })
+  })
+
+  test.describe('no preference for reduced motion', () => {
+    test.use({contextOptions: {reducedMotion: 'no-preference'}})
+
+    test('keeps the hover transition @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'components-avatarstack-features--align-left',
+      })
+
+      const {transitionDuration, transitionProperty} = await describeTransition(page)
+      expect(transitionProperty).toContain('margin')
+      expect(transitionDuration).toBe('0.2s, 0.2s, 0.2s, 0.2s')
+    })
+  })
 })
diff --git a/packages/react/src/AvatarStack/AvatarStack.module.css b/packages/react/src/AvatarStack/AvatarStack.module.css
index e6c77986b84..09d7627c6de 100644
--- a/packages/react/src/AvatarStack/AvatarStack.module.css
+++ b/packages/react/src/AvatarStack/AvatarStack.module.css
@@ -132,11 +132,18 @@
   height: var(--avatar-stack-size);
   overflow: hidden;
   flex-shrink: 0;
-  transition:
-    margin 0.2s ease-in-out,
-    opacity 0.2s ease-in-out,
-    mask-position 0.2s ease-in-out,
-    mask-size 0.2s ease-in-out;
+
+  /* The hover reveal slides each avatar across the screen, so it is opt-in like
+     the rest of the library (Skeleton, ProgressBar, SegmentedControl). The
+     expanded positions come from the `:hover` rules below, so with motion
+     reduced every avatar is still revealed - it just happens immediately. */
+  @media (prefers-reduced-motion: no-preference) {
+    transition:
+      margin 0.2s ease-in-out,
+      opacity 0.2s ease-in-out,
+      mask-position 0.2s ease-in-out,
+      mask-size 0.2s ease-in-out;
+  }
 
   /* stylelint-disable-next-line selector-no-qualifying-type */
   .AvatarStack:where([data-shape='circle']) &:is(img) {