diff --git a/.changeset/actionlist-checkmark-reduced-motion.md b/.changeset/actionlist-checkmark-reduced-motion.md
new file mode 100644
index 00000000000..bcf43388da5
--- /dev/null
+++ b/.changeset/actionlist-checkmark-reduced-motion.md
@@ -0,0 +1,5 @@
+---
+'@primer/react': patch
+---
+
+ActionList: Respect prefers-reduced-motion for the multi-select checkmark animation.
diff --git a/e2e/components/ActionList.test.ts b/e2e/components/ActionList.test.ts
index 2cf513506eb..2bc074e265a 100644
--- a/e2e/components/ActionList.test.ts
+++ b/e2e/components/ActionList.test.ts
@@ -216,4 +216,74 @@ test.describe('ActionList', () => {
       })
     }
   })
+
+  // A multi-select checkbox only renders when the list is not a `menu`:
+  // `Selection` falls back to the single-select checkmark for `role="menu"`, so
+  // the FilteredActionList story - a `role="listbox"` with
+  // `selectionVariant="multiple"` - is what produces `.MultiSelectCheckbox`.
+  const bugItem = (page: Page) => page.getByRole('option', {name: /^bug/})
+
+  const describeCheckmark = (page: Page) =>
+    bugItem(page)
+      .locator('[data-component="ActionList.Selection"] > div')
+      .evaluate(element => {
+        const {animationName, clipPath, visibility, transitionDelay} = getComputedStyle(element, '::before')
+        return {animationName, clipPath, visibility, transitionDelay}
+      })
+
+  test.describe('reduced motion', () => {
+    // `contextOptions` is what the root Playwright config uses to emulate
+    // `prefers-reduced-motion`, so it is the option that has to be overridden.
+    test.use({contextOptions: {reducedMotion: 'reduce'}})
+
+    test('checkmark is shown without the clip-path animation @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'components-filteredactionlist-features--multi-select-with-select-all',
+      })
+
+      // `bug` is one of the two items the story starts out with selected
+      expect(await describeCheckmark(page)).toEqual({
+        animationName: 'none',
+        // Chromium reports a fully un-clipped value as `inset(0px)`
+        clipPath: 'inset(0px)',
+        visibility: 'visible',
+        transitionDelay: '0s',
+      })
+
+      // Clearing the selection hides the mark straight away - the wipe-out and
+      // the 230ms `visibility` delay that goes with it are both motion-only
+      await bugItem(page).click()
+
+      expect(await describeCheckmark(page)).toEqual({
+        animationName: 'none',
+        clipPath: 'inset(16px 0px 0px)',
+        visibility: 'hidden',
+        transitionDelay: '0s',
+      })
+    })
+  })
+
+  test.describe('no preference for reduced motion', () => {
+    test.use({contextOptions: {reducedMotion: 'no-preference'}})
+
+    test('checkmark keeps its clip-path animation @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'components-filteredactionlist-features--multi-select-with-select-all',
+      })
+
+      // Names are scoped by CSS Modules, so only the base name is stable. The
+      // clip-path is not worth asserting here: by the time it can be read, the
+      // 80ms wipe has already landed on the un-clipped value.
+      const checkmark = await describeCheckmark(page)
+      expect(checkmark.animationName).toMatch(/checkmarkIn/)
+      expect(checkmark.visibility).toBe('visible')
+
+      await bugItem(page).click()
+
+      const cleared = await describeCheckmark(page)
+      expect(cleared.animationName).toMatch(/checkmarkOut/)
+      // The mark is kept visible until the wipe-out has run
+      expect(cleared.transitionDelay).toBe('0.23s')
+    })
+  })
 })
diff --git a/packages/react/src/ActionList/ActionList.module.css b/packages/react/src/ActionList/ActionList.module.css
index 7a4a9065c0a..8ece6a80cfa 100644
--- a/packages/react/src/ActionList/ActionList.module.css
+++ b/packages/react/src/ActionList/ActionList.module.css
@@ -427,7 +427,6 @@
       content: '';
       /* stylelint-disable-next-line primer/colors */
       background-color: var(--control-checked-fgColor-rest);
-      transition: visibility 0s linear 230ms;
       clip-path: inset(var(--base-size-16) 0 0 0);
 
       /* octicon checkmark image */
@@ -435,8 +434,15 @@
       mask-size: 75%;
       mask-repeat: no-repeat;
       mask-position: center;
-      animation: checkmarkOut 80ms cubic-bezier(0.65, 0, 0.35, 1);
-      /*  forwards;  slightly snappier animation out */
+
+      /* The clip-path wipe is decorative, so it is opt-in like the rest of the
+         library (Checkbox, Skeleton, SegmentedControl). */
+      @media (prefers-reduced-motion: no-preference) {
+        /* checked -> unchecked - wait out the wipe-out before hiding the mark */
+        transition: visibility 0s linear 230ms;
+        animation: checkmarkOut 80ms cubic-bezier(0.65, 0, 0.35, 1);
+        /*  forwards;  slightly snappier animation out */
+      }
     }
 
     @media (forced-colors: active) {
@@ -459,8 +465,15 @@
 
       &::before {
         visibility: visible;
-        transition: visibility 0s linear 0s;
-        animation: checkmarkIn 80ms cubic-bezier(0.65, 0, 0.35, 1) forwards 80ms;
+        /* Reduced motion: the mark is simply present, without the wipe */
+        clip-path: inset(0 0 0 0);
+
+        @media (prefers-reduced-motion: no-preference) {
+          transition: visibility 0s linear 0s;
+          /* Keep the mark clipped while `checkmarkIn` waits out its 80ms delay */
+          clip-path: inset(var(--base-size-16) 0 0 0);
+          animation: checkmarkIn 80ms cubic-bezier(0.65, 0, 0.35, 1) forwards 80ms;
+        }
       }
 
       @media (forced-colors: active) {