diff --git a/.changeset/checkbox-reduced-motion.md b/.changeset/checkbox-reduced-motion.md
new file mode 100644
index 00000000000..916383b84a3
--- /dev/null
+++ b/.changeset/checkbox-reduced-motion.md
@@ -0,0 +1,5 @@
+---
+'@primer/react': patch
+---
+
+Checkbox: Respect prefers-reduced-motion for the checkmark animation.
diff --git a/e2e/components/Checkbox.test.ts b/e2e/components/Checkbox.test.ts
index 68bdc98193f..1b45ca9d12f 100644
--- a/e2e/components/Checkbox.test.ts
+++ b/e2e/components/Checkbox.test.ts
@@ -1,4 +1,4 @@
-import {test, expect} from '@playwright/test'
+import {test, expect, type Page} from '@playwright/test'
 import {visit} from '../test-helpers/storybook'
 import {themes} from '../test-helpers/themes'
 
@@ -72,4 +72,79 @@ test.describe('Checkbox', () => {
       }
     })
   }
+
+  // The checkmark is drawn by the input's `::before`, which `toHaveCSS` cannot
+  // target, so these read the computed style of the pseudo-element directly.
+  const describeCheckmark = (page: Page) =>
+    page.locator('[data-component="Checkbox"]').evaluate(element => {
+      const {animationName, clipPath, visibility, transitionDelay} = getComputedStyle(element, '::before')
+      return {animationName, clipPath, visibility, transitionDelay}
+    })
+
+  test.describe('reduced motion', () => {
+    // `contextOptions` is what the root Playwright config uses to emulate
+    // `prefers-reduced-motion`, so it is the option that has to be overridden.
+    test.use({contextOptions: {reducedMotion: 'reduce'}})
+
+    test('checkmark appears without the clip-path animation @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'components-checkbox--playground',
+        args: {checked: true},
+      })
+
+      expect(await describeCheckmark(page)).toEqual({
+        animationName: 'none',
+        // Chromium reports a fully un-clipped value as `inset(0px)`
+        clipPath: 'inset(0px)',
+        visibility: 'visible',
+        transitionDelay: '0s',
+      })
+
+      await visit(page, {
+        id: 'components-checkbox-features--indeterminate',
+      })
+
+      expect(await describeCheckmark(page)).toEqual({
+        animationName: 'none',
+        clipPath: 'inset(0px)',
+        visibility: 'visible',
+        transitionDelay: '0s',
+      })
+
+      // Clearing the indeterminate state hides the mark immediately, with no
+      // delay left over from the animation-out
+      await page.locator('[data-component="Checkbox"]').click()
+
+      expect(await describeCheckmark(page)).toEqual({
+        animationName: 'none',
+        clipPath: 'inset(16px 0px 0px)',
+        visibility: 'hidden',
+        transitionDelay: '0s',
+      })
+    })
+  })
+
+  test.describe('no preference for reduced motion', () => {
+    test.use({contextOptions: {reducedMotion: 'no-preference'}})
+
+    test('checkmark keeps its clip-path animation @vrt', async ({page}) => {
+      await visit(page, {
+        id: 'components-checkbox-features--indeterminate',
+      })
+
+      // Names are scoped by CSS Modules, so only the base name is stable. The
+      // clip-path itself is not worth asserting here: by the time it can be
+      // read, the 80ms wipe has already run and landed on the un-clipped value.
+      const checkmark = await describeCheckmark(page)
+      expect(checkmark.animationName).toMatch(/checkmarkIn/)
+      expect(checkmark.visibility).toBe('visible')
+
+      await page.locator('[data-component="Checkbox"]').click()
+
+      const cleared = await describeCheckmark(page)
+      expect(cleared.animationName).toMatch(/checkmarkOut/)
+      // The mark is kept visible until the wipe-out has run
+      expect(cleared.transitionDelay).toBe('0.23s')
+    })
+  })
 })
diff --git a/packages/react/src/Checkbox/Checkbox.module.css b/packages/react/src/Checkbox/Checkbox.module.css
index 26e48109d40..9ae48547214 100644
--- a/packages/react/src/Checkbox/Checkbox.module.css
+++ b/packages/react/src/Checkbox/Checkbox.module.css
@@ -13,13 +13,19 @@
     content: '';
     /* stylelint-disable-next-line primer/colors */
     background-color: var(--fgColor-onEmphasis);
-    transition: visibility 0s linear 230ms;
     clip-path: inset(var(--base-size-16) 0 0 0);
     mask-image: url('data:image/svg+xml;base64,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');
     mask-size: 75%;
     mask-repeat: no-repeat;
     mask-position: center;
-    animation: checkmarkOut 80ms cubic-bezier(0.65, 0, 0.35, 1) forwards;
+
+    /* The clip-path wipe is decorative, so it is opt-in like every other
+       animated component (Skeleton, SegmentedControl). */
+    @media (prefers-reduced-motion: no-preference) {
+      /* checked -> unchecked - wait out the wipe-out before hiding the mark */
+      transition: visibility 0s linear 230ms;
+      animation: checkmarkOut 80ms cubic-bezier(0.65, 0, 0.35, 1) forwards;
+    }
   }
 
   &:checked,
@@ -39,7 +45,14 @@
 
   &:checked::before,
   &:indeterminate::before {
-    animation: checkmarkIn 80ms cubic-bezier(0.65, 0, 0.35, 1) forwards 80ms;
+    /* Reduced motion: the mark is simply present, without the wipe */
+    clip-path: inset(0 0 0 0);
+
+    @media (prefers-reduced-motion: no-preference) {
+      /* Keep the mark clipped while `checkmarkIn` waits out its 80ms delay */
+      clip-path: inset(var(--base-size-16) 0 0 0);
+      animation: checkmarkIn 80ms cubic-bezier(0.65, 0, 0.35, 1) forwards 80ms;
+    }
   }
 
   &:checked:disabled,