diff --git a/tests/functional/config.py b/tests/functional/config.py
index 9083668f7c..f2429d6f83 100644
--- a/tests/functional/config.py
+++ b/tests/functional/config.py
@@ -186,6 +186,9 @@ def get_uses_mapping():
 infer_timeouts = {
     TargetDevice.CPU: default_infer_timeout,
     TargetDevice.GPU: default_gpu_infer_timeout,
+    TargetDevice.GPU_0: default_gpu_infer_timeout,
+    TargetDevice.GPU_1: default_gpu_infer_timeout,
+    TargetDevice.GPU_2: default_gpu_infer_timeout,
     TargetDevice.NPU: default_npu_infer_timeout,
     TargetDevice.AUTO: default_gpu_infer_timeout,
     TargetDevice.HETERO: default_gpu_infer_timeout,
diff --git a/tests/functional/constants/target_device.py b/tests/functional/constants/target_device.py
index 8d06e0f0df..55d83c11b7 100644
--- a/tests/functional/constants/target_device.py
+++ b/tests/functional/constants/target_device.py
@@ -20,6 +20,9 @@
 class TargetDevice:
     CPU = "CPU"
     GPU = "GPU"
+    GPU_0 = "GPU:0"
+    GPU_1 = "GPU:1"
+    GPU_2 = "GPU:2"
     NPU = "NPU"
     AUTO = "AUTO:GPU,CPU"
     HETERO = "HETERO:GPU,CPU"
diff --git a/tests/functional/utils/helpers.py b/tests/functional/utils/helpers.py
index 5e9ffbae1d..81b1d93833 100644
--- a/tests/functional/utils/helpers.py
+++ b/tests/functional/utils/helpers.py
@@ -95,9 +95,27 @@ def get_multi_target_devices(target_devices_list, separator):
     return result
 
 
+def _is_device_with_index(device_str):
+    """ Check if device string is a device with numeric index, e.g. GPU:0, GPU:1. """
+    if ":" in device_str:
+        _, suffix = device_str.split(":", 1)
+        return suffix.isdigit()
+    return False
+
+
 def validate_supported_values(detected_list, supported_list):
     supported_list += ALL_AVAILABLE_OPTIONS  # 'starred expression' will be evaluated during pytest_configure
-    check = all(_elem in supported_list for _elem in detected_list)
+
+    def _is_supported(device):
+        if device in supported_list:
+            return True
+        # Accept indexed devices like GPU:0, GPU:1 if base device (GPU) is supported
+        if _is_device_with_index(device):
+            base_device = device.split(":", 1)[0]
+            return base_device in supported_list
+        return False
+
+    check = all(_is_supported(_elem) for _elem in detected_list)
     assert check, f"Not supported target devices in {detected_list}"
     return detected_list
 
@@ -106,7 +124,12 @@ def get_target_devices():
     """ Convert comma separated string of devices into list """
     target_devices_list = get_list("TT_TARGET_DEVICE", fallback=[TargetDevice.CPU])
     separator_multi = ":"
-    if any(separator_multi in _target_device for _target_device in target_devices_list):
+    # Only treat as multi-target if ':' is followed by a device name, not a numeric index (GPU:0, GPU:1)
+    has_multi_target = any(
+        separator_multi in _td and not _is_device_with_index(_td)
+        for _td in target_devices_list
+    )
+    if has_multi_target:
         target_devices_list = get_multi_target_devices(target_devices_list, separator_multi)
     ov_target_devices = [value for key, value in vars(TargetDevice).items() if not key.startswith("__")]
     target_devices_list = validate_supported_values(detected_list=target_devices_list, supported_list=ov_target_devices)