diff --git a/change/@fluentui-react-4beba3a6-0d78-4d2a-a593-a4b1d998a4d9.json b/change/@fluentui-react-4beba3a6-0d78-4d2a-a593-a4b1d998a4d9.json
new file mode 100644
index 00000000000000..cdae7151914cde
--- /dev/null
+++ b/change/@fluentui-react-4beba3a6-0d78-4d2a-a593-a4b1d998a4d9.json
@@ -0,0 +1,7 @@
+{
+  "type": "none",
+  "comment": "Shows TextField value as title attribute when disabled/read-only and truncated",
+  "packageName": "@fluentui/react",
+  "email": "tomas.blazek@brose.com",
+  "dependentChangeType": "none"
+}
diff --git a/packages/react/src/components/TextField/TextField.base.tsx b/packages/react/src/components/TextField/TextField.base.tsx
index aac22450187717..e90392ff99603b 100644
--- a/packages/react/src/components/TextField/TextField.base.tsx
+++ b/packages/react/src/components/TextField/TextField.base.tsx
@@ -133,6 +133,7 @@ export class TextFieldBase
 
   public componentDidMount(): void {
     this._adjustInputHeight();
+    this._updateTitleAttribute();
 
     if (this.props.validateOnLoad) {
       this._validate(this.value);
@@ -196,6 +197,8 @@ export class TextFieldBase
         this._delayedValidate(value);
       }
     }
+
+    this._updateTitleAttribute();
   }
 
   public render(): JSXElement {
@@ -655,6 +658,38 @@ export class TextFieldBase
       }
     }
   }
+
+  private _updateTitleAttribute(): void {
+    const textElement = this._textElement.current;
+    const value = this.value;
+    const providedTitle = this.props.title;
+
+    if (!textElement) {
+      return;
+    }
+
+    // Always honor an explicitly provided title prop.
+    if (providedTitle !== undefined) {
+      textElement.title = providedTitle;
+      return;
+    }
+
+    if (
+      this.props.multiline ||
+      (!this.props.disabled && !this.props.readOnly) ||
+      !value ||
+      !this._isTextElementOverflowing(textElement)
+    ) {
+      textElement.removeAttribute('title');
+      return;
+    }
+
+    textElement.title = value;
+  }
+
+  private _isTextElementOverflowing(textElement: HTMLTextAreaElement | HTMLInputElement): boolean {
+    return textElement.scrollWidth > textElement.clientWidth;
+  }
 }
 
 /** Get the value from the given state and props (converting from number to string if needed) */
diff --git a/packages/react/src/components/TextField/TextField.test.tsx b/packages/react/src/components/TextField/TextField.test.tsx
index c54b5e01843311..e68075ca2104b8 100644
--- a/packages/react/src/components/TextField/TextField.test.tsx
+++ b/packages/react/src/components/TextField/TextField.test.tsx
@@ -25,6 +25,17 @@ const textFieldRef: IRefObject<ITextField> = (ref: ITextField | null) => {
 
 const noOp = () => undefined;
 
+function setOverflow(element: HTMLElement, scrollWidth: number, clientWidth: number) {
+  Object.defineProperty(element, 'scrollWidth', {
+    configurable: true,
+    value: scrollWidth,
+  });
+  Object.defineProperty(element, 'clientWidth', {
+    configurable: true,
+    value: clientWidth,
+  });
+}
+
 function sharedBeforeEach() {
   resetIds();
   resetControlledWarnings();
@@ -267,6 +278,61 @@ describe('TextField basic props', () => {
     const input = getByRole('textbox') as HTMLInputElement;
     expect(input.readOnly).toEqual(true);
   });
+  it('should apply title with the value when disabled and truncated', () => {
+    const value = 'some very long text that should overflow';
+    const { getByRole, rerender } = render(<TextField disabled value={value} onChange={noOp} />);
+    const input = getByRole('textbox') as HTMLInputElement;
+
+    setOverflow(input, 200, 100);
+    rerender(<TextField disabled value={value} onChange={noOp} />);
+
+    expect(input.title).toEqual(value);
+  });
+  it('should not apply title when disabled and not truncated', () => {
+    const value = 'short text';
+    const { getByRole, rerender } = render(<TextField disabled value={value} onChange={noOp} />);
+    const input = getByRole('textbox') as HTMLInputElement;
+
+    setOverflow(input, 100, 100);
+    rerender(<TextField disabled value={value} onChange={noOp} />);
+
+    expect(input.title).toEqual('');
+  });
+  it('should apply title with the value when readOnly and truncated', () => {
+    const value = 'some very long text that should overflow';
+    const { getByRole, rerender } = render(<TextField readOnly value={value} onChange={noOp} />);
+    const input = getByRole('textbox') as HTMLInputElement;
+
+    setOverflow(input, 200, 100);
+    rerender(<TextField readOnly value={value} onChange={noOp} />);
+
+    expect(input.title).toEqual(value);
+  });
+  it('should not apply title when readOnly and not truncated', () => {
+    const value = 'short text';
+    const { getByRole, rerender } = render(<TextField readOnly value={value} onChange={noOp} />);
+    const input = getByRole('textbox') as HTMLInputElement;
+
+    setOverflow(input, 100, 100);
+    rerender(<TextField readOnly value={value} onChange={noOp} />);
+
+    expect(input.title).toEqual('');
+  });
+  it('should not auto-apply title for multiline fields', () => {
+    const value = 'some very long text that should overflow';
+    const { getByRole } = render(<TextField multiline value={value} onChange={noOp} />);
+    const input = getByRole('textbox') as HTMLTextAreaElement;
+
+    setOverflow(input, 200, 100);
+
+    expect(input.title).toEqual('');
+  });
+  it('should preserve an explicitly provided title prop', () => {
+    const { getByRole } = render(<TextField multiline title="custom title" value="value" onChange={noOp} />);
+    const input = getByRole('textbox') as HTMLTextAreaElement;
+
+    expect(input.title).toEqual('custom title');
+  });
   it('can render description text', () => {
     const testDescription = 'A custom description';
     const { container } = render(<TextField description={testDescription} />);