diff --git a/src/wp-includes/script-loader.php b/src/wp-includes/script-loader.php
index fd1f24a08968d..ac8063e7d256c 100644
--- a/src/wp-includes/script-loader.php
+++ b/src/wp-includes/script-loader.php
@@ -1099,7 +1099,6 @@ function wp_default_scripts( $scripts ) {
 					'language' => strtolower( strtok( determine_locale(), '_-' ) ),
 					'strings'  => array(
 						'mejs.download-file'       => __( 'Download File' ),
-						'mejs.install-flash'       => __( 'You are using a browser that does not have Flash player enabled or installed. Please turn on your Flash player plugin or download the latest version from https://get.adobe.com/flashplayer/' ),
 						'mejs.fullscreen'          => __( 'Fullscreen' ),
 						'mejs.play'                => __( 'Play' ),
 						'mejs.pause'               => __( 'Pause' ),
diff --git a/tests/phpunit/tests/dependencies/scripts.php b/tests/phpunit/tests/dependencies/scripts.php
index bbf301fe424cd..3233bc2e61471 100644
--- a/tests/phpunit/tests/dependencies/scripts.php
+++ b/tests/phpunit/tests/dependencies/scripts.php
@@ -1776,6 +1776,23 @@ public function test_priority_of_dependency_for_non_enqueued_dependent() {
 		);
 	}
 
+	/**
+	 * Tests that the obsolete Flash-related MediaElement.js string is no longer localized.
+	 *
+	 * @ticket 65737
+	 *
+	 * @covers ::wp_default_scripts
+	 */
+	public function test_mediaelement_l10n_does_not_include_flash_message() {
+		$wp_scripts = wp_scripts();
+		wp_default_scripts( $wp_scripts );
+
+		$inline_script = implode( '', (array) $wp_scripts->get_data( 'mediaelement-core', 'before' ) );
+
+		$this->assertStringNotContainsString( 'mejs.install-flash', $inline_script );
+		$this->assertStringContainsString( 'mejs.play', $inline_script );
+	}
+
 	/**
 	 * Tests that printing a script without enqueueing has the same output as when it is enqueued.
 	 *