diff --git a/Source/Testably.Abstractions.Testing/FileSystemInitializerExtensions.cs b/Source/Testably.Abstractions.Testing/FileSystemInitializerExtensions.cs
index 5113b2a5..ca479543 100644
--- a/Source/Testably.Abstractions.Testing/FileSystemInitializerExtensions.cs
+++ b/Source/Testably.Abstractions.Testing/FileSystemInitializerExtensions.cs
@@ -108,6 +108,40 @@ public static void InitializeEmbeddedResourcesFromAssembly(this IFileSystem file
 	}
 	#pragma warning restore MA0051 // Method is too long
 
+	/// <summary>
+	///     Initializes the <see cref="IFileSystem" /> from the real <paramref name="sourceDirectory" /> into the
+	///     <paramref name="targetDirectory" /> on the provided file system.
+	/// </summary>
+	/// <param name="fileSystem">The target file system to which the data is copied.</param>
+	/// <param name="sourceDirectory">
+	///     The source directory on the real file system that is copied to the
+	///     <paramref name="fileSystem" />.
+	/// </param>
+	/// <param name="targetDirectory">
+	///     The target directory on the <paramref name="fileSystem" /> to which the data is copied to.<br />
+	///     If no <paramref name="targetDirectory" /> is set, <paramref name="sourceDirectory" /> is used instead.
+	/// </param>
+	/// <remarks>
+	///     <b>Warning</b>:<br />
+	///     This method will recursively copy the content of all files and directories from the
+	///     <paramref name="sourceDirectory" /> to the <paramref name="targetDirectory" />. With large files, this can be very
+	///     resource intensive!<br />
+	/// </remarks>
+	public static void InitializeFromRealDirectory(this IFileSystem fileSystem,
+		string sourceDirectory, string? targetDirectory = null)
+	{
+		using IDisposable release = fileSystem.IgnoreStatistics();
+		targetDirectory ??= sourceDirectory;
+		if (fileSystem.Path.IsPathRooted(targetDirectory) &&
+		    fileSystem is MockFileSystem mockFileSystem)
+		{
+			string? drive = fileSystem.Path.GetPathRoot(targetDirectory);
+			mockFileSystem.WithDrive(drive);
+		}
+
+		CopyDirectory(fileSystem, sourceDirectory, targetDirectory);
+	}
+
 	/// <summary>
 	///     Initializes the <see cref="IFileSystem" /> in the <paramref name="basePath" /> with test data.
 	/// </summary>
@@ -158,6 +192,31 @@ public static IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(
 		return new DirectoryCleaner(fileSystem, prefix, logger);
 	}
 
+	private static void CopyDirectory(
+		IFileSystem fileSystem, string sourceDirectory, string targetDirectory)
+	{
+		if (!Directory.Exists(sourceDirectory))
+		{
+			throw new DirectoryNotFoundException(
+				$"The directory '{sourceDirectory}' does not exist.");
+		}
+
+		fileSystem.Directory.CreateDirectory(targetDirectory);
+		foreach (string file in Directory.EnumerateFiles(sourceDirectory))
+		{
+			string fileName = Path.GetFileName(file);
+			fileSystem.File.WriteAllBytes(fileSystem.Path.Combine(targetDirectory, fileName),
+				File.ReadAllBytes(file));
+		}
+
+		foreach (string directory in Directory.EnumerateDirectories(sourceDirectory))
+		{
+			string directoryName = Path.GetFileName(directory);
+			CopyDirectory(fileSystem, directory,
+				fileSystem.Path.Combine(targetDirectory, directoryName));
+		}
+	}
+
 	private static void InitializeFileFromEmbeddedResource(this IFileSystem fileSystem,
 		string path,
 		Assembly assembly,
diff --git a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net10.0.txt b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net10.0.txt
index 8219bb8e..921b4f81 100644
--- a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net10.0.txt
+++ b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net10.0.txt
@@ -18,6 +18,7 @@ namespace Testably.Abstractions.Testing
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> Initialize<TFileSystem>(this TFileSystem fileSystem, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static void InitializeEmbeddedResourcesFromAssembly(this System.IO.Abstractions.IFileSystem fileSystem, string directoryPath, System.Reflection.Assembly assembly, string? relativePath = null, string searchPattern = "*", System.IO.SearchOption searchOption = 1) { }
+        public static void InitializeFromRealDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string sourceDirectory, string? targetDirectory = null) { }
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> InitializeIn<TFileSystem>(this TFileSystem fileSystem, string basePath, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static Testably.Abstractions.Testing.Initializer.IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string? prefix = null, System.Action<string>? logger = null) { }
diff --git a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net6.0.txt b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net6.0.txt
index e9819ffe..8891e371 100644
--- a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net6.0.txt
+++ b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net6.0.txt
@@ -18,6 +18,7 @@ namespace Testably.Abstractions.Testing
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> Initialize<TFileSystem>(this TFileSystem fileSystem, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static void InitializeEmbeddedResourcesFromAssembly(this System.IO.Abstractions.IFileSystem fileSystem, string directoryPath, System.Reflection.Assembly assembly, string? relativePath = null, string searchPattern = "*", System.IO.SearchOption searchOption = 1) { }
+        public static void InitializeFromRealDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string sourceDirectory, string? targetDirectory = null) { }
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> InitializeIn<TFileSystem>(this TFileSystem fileSystem, string basePath, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static Testably.Abstractions.Testing.Initializer.IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string? prefix = null, System.Action<string>? logger = null) { }
diff --git a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net8.0.txt b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net8.0.txt
index a8ed9873..7ade5f59 100644
--- a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net8.0.txt
+++ b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net8.0.txt
@@ -18,6 +18,7 @@ namespace Testably.Abstractions.Testing
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> Initialize<TFileSystem>(this TFileSystem fileSystem, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static void InitializeEmbeddedResourcesFromAssembly(this System.IO.Abstractions.IFileSystem fileSystem, string directoryPath, System.Reflection.Assembly assembly, string? relativePath = null, string searchPattern = "*", System.IO.SearchOption searchOption = 1) { }
+        public static void InitializeFromRealDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string sourceDirectory, string? targetDirectory = null) { }
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> InitializeIn<TFileSystem>(this TFileSystem fileSystem, string basePath, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static Testably.Abstractions.Testing.Initializer.IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string? prefix = null, System.Action<string>? logger = null) { }
diff --git a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net9.0.txt b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net9.0.txt
index 94c965e1..ff084f74 100644
--- a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net9.0.txt
+++ b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_net9.0.txt
@@ -18,6 +18,7 @@ namespace Testably.Abstractions.Testing
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> Initialize<TFileSystem>(this TFileSystem fileSystem, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static void InitializeEmbeddedResourcesFromAssembly(this System.IO.Abstractions.IFileSystem fileSystem, string directoryPath, System.Reflection.Assembly assembly, string? relativePath = null, string searchPattern = "*", System.IO.SearchOption searchOption = 1) { }
+        public static void InitializeFromRealDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string sourceDirectory, string? targetDirectory = null) { }
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> InitializeIn<TFileSystem>(this TFileSystem fileSystem, string basePath, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static Testably.Abstractions.Testing.Initializer.IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string? prefix = null, System.Action<string>? logger = null) { }
diff --git a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.0.txt b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.0.txt
index 1a67f362..e5020493 100644
--- a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.0.txt
+++ b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.0.txt
@@ -14,6 +14,7 @@ namespace Testably.Abstractions.Testing
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> Initialize<TFileSystem>(this TFileSystem fileSystem, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static void InitializeEmbeddedResourcesFromAssembly(this System.IO.Abstractions.IFileSystem fileSystem, string directoryPath, System.Reflection.Assembly assembly, string? relativePath = null, string searchPattern = "*", System.IO.SearchOption searchOption = 1) { }
+        public static void InitializeFromRealDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string sourceDirectory, string? targetDirectory = null) { }
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> InitializeIn<TFileSystem>(this TFileSystem fileSystem, string basePath, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static Testably.Abstractions.Testing.Initializer.IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string? prefix = null, System.Action<string>? logger = null) { }
diff --git a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.1.txt b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.1.txt
index 95c397d7..ebd68b1f 100644
--- a/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.1.txt
+++ b/Tests/Api/Testably.Abstractions.Api.Tests/Expected/Testably.Abstractions.Testing_netstandard2.1.txt
@@ -14,6 +14,7 @@ namespace Testably.Abstractions.Testing
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> Initialize<TFileSystem>(this TFileSystem fileSystem, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static void InitializeEmbeddedResourcesFromAssembly(this System.IO.Abstractions.IFileSystem fileSystem, string directoryPath, System.Reflection.Assembly assembly, string? relativePath = null, string searchPattern = "*", System.IO.SearchOption searchOption = 1) { }
+        public static void InitializeFromRealDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string sourceDirectory, string? targetDirectory = null) { }
         public static Testably.Abstractions.Testing.Initializer.IFileSystemInitializer<TFileSystem> InitializeIn<TFileSystem>(this TFileSystem fileSystem, string basePath, System.Action<Testably.Abstractions.Testing.FileSystemInitializerOptions>? options = null)
             where TFileSystem : System.IO.Abstractions.IFileSystem { }
         public static Testably.Abstractions.Testing.Initializer.IDirectoryCleaner SetCurrentDirectoryToEmptyTemporaryDirectory(this System.IO.Abstractions.IFileSystem fileSystem, string? prefix = null, System.Action<string>? logger = null) { }
diff --git a/Tests/Testably.Abstractions.Testing.Tests/FileSystemInitializerExtensionsTests.cs b/Tests/Testably.Abstractions.Testing.Tests/FileSystemInitializerExtensionsTests.cs
index aaa1cccc..70859a22 100644
--- a/Tests/Testably.Abstractions.Testing.Tests/FileSystemInitializerExtensionsTests.cs
+++ b/Tests/Testably.Abstractions.Testing.Tests/FileSystemInitializerExtensionsTests.cs
@@ -1,4 +1,5 @@
-using System.Collections.Generic;
+using aweXpect.Testably;
+using System.Collections.Generic;
 using System.IO;
 using System.Linq;
 using System.Reflection;
@@ -243,6 +244,108 @@ await That(result)
 			.Contains(x => x.EndsWith("SubResourceFile1.txt", StringComparison.Ordinal));
 	}
 
+	[Theory]
+	[AutoData]
+	public async Task InitializeFromRealDirectory_MissingDrive_ShouldCreateDrive(
+		string directoryName)
+	{
+		Skip.IfNot(Test.RunsOnWindows);
+
+		string tempPath = Path.Combine(Path.GetTempPath(), Path.GetRandomFileName());
+		try
+		{
+			Directory.CreateDirectory(tempPath);
+			MockFileSystem sut = new();
+			IDriveInfo[] drives = sut.DriveInfo.GetDrives();
+			for (char c = 'D'; c <= 'Z'; c++)
+			{
+				if (drives.Any(d => d.Name.StartsWith($"{c}", StringComparison.Ordinal)))
+				{
+					continue;
+				}
+
+				directoryName = Path.Combine($"{c}:\\", directoryName);
+				break;
+			}
+
+			sut.InitializeFromRealDirectory(tempPath, directoryName);
+
+			await That(sut.Directory.Exists(directoryName)).IsTrue();
+			await That(sut.DriveInfo.GetDrives()).HasCount(drives.Length + 1);
+		}
+		finally
+		{
+			Directory.Delete(tempPath, true);
+		}
+	}
+
+	[Fact]
+	public async Task InitializeFromRealDirectory_ShouldCopyFileToTargetDirectory()
+	{
+		MockFileSystem fileSystem = new();
+		string tempPath = Path.Combine(Path.GetTempPath(), Path.GetRandomFileName());
+		try
+		{
+			Directory.CreateDirectory(tempPath);
+			string filePath = Path.Combine(tempPath, "test.txt");
+			File.WriteAllText(filePath, "Hello, World!");
+
+			fileSystem.InitializeFromRealDirectory(tempPath, "foo");
+
+			await That(fileSystem).HasDirectory("foo");
+			await That(fileSystem).HasFile("foo/test.txt").WithContent("Hello, World!");
+		}
+		finally
+		{
+			Directory.Delete(tempPath, true);
+		}
+	}
+
+	[Fact]
+	public async Task
+		InitializeFromRealDirectory_ShouldRecursivelyCopyDirectoriesToTargetDirectory()
+	{
+		MockFileSystem fileSystem = new();
+		string tempPath = Path.Combine(Path.GetTempPath(), Path.GetRandomFileName());
+		try
+		{
+			Directory.CreateDirectory(tempPath);
+			Directory.CreateDirectory(Path.Combine(tempPath, "subdir"));
+			File.WriteAllText(Path.Combine(tempPath, "subdir", "test1.txt"), "foo");
+			File.WriteAllText(Path.Combine(tempPath, "subdir", "test2.txt"), "bar");
+
+			fileSystem.InitializeFromRealDirectory(tempPath);
+
+			await That(fileSystem).HasDirectory(fileSystem.Path.Combine(tempPath, "subdir"));
+			await That(fileSystem).HasFile(fileSystem.Path.Combine(tempPath, "subdir", "test1.txt"))
+				.WithContent("foo");
+			await That(fileSystem).HasFile(fileSystem.Path.Combine(tempPath, "subdir", "test2.txt"))
+				.WithContent("bar");
+		}
+		finally
+		{
+			Directory.Delete(tempPath, true);
+		}
+	}
+
+	[Fact]
+	public async Task
+		InitializeFromRealDirectory_WhenDirectoryDoesNotExist_ShouldThrowDirectoryNotFoundException()
+	{
+		MockFileSystem fileSystem = new();
+		string tempPath = Path.Combine(Path.GetTempPath(), Path.GetRandomFileName());
+		while (Directory.Exists(tempPath))
+		{
+			tempPath = Path.Combine(Path.GetTempPath(), Path.GetRandomFileName());
+		}
+
+		void Act()
+			=> fileSystem.InitializeFromRealDirectory(tempPath, "foo");
+
+		await That(Act).Throws<DirectoryNotFoundException>()
+			.WithMessage($"The directory '{tempPath}' does not exist.");
+	}
+
 	[Theory]
 	[AutoData]
 	public async Task InitializeIn_MissingDrive_ShouldCreateDrive(string directoryName)