diff --git a/pathology-api/poetry.lock b/pathology-api/poetry.lock
index 49744d3..61aa14e 100644
--- a/pathology-api/poetry.lock
+++ b/pathology-api/poetry.lock
@@ -2176,14 +2176,14 @@ six = ">=1.5"
 
 [[package]]
 name = "python-dotenv"
-version = "1.2.1"
+version = "1.2.2"
 description = "Read key-value pairs from a .env file and set them as environment variables"
 optional = false
-python-versions = ">=3.9"
+python-versions = ">=3.10"
 groups = ["dev"]
 files = [
-    {file = "python_dotenv-1.2.1-py3-none-any.whl", hash = "sha256:b81ee9561e9ca4004139c6cbba3a238c32b03e4894671e181b671e8cb8425d61"},
-    {file = "python_dotenv-1.2.1.tar.gz", hash = "sha256:42667e897e16ab0d66954af0e60a9caa94f0fd4ecf3aaf6d2d260eec1aa36ad6"},
+    {file = "python_dotenv-1.2.2-py3-none-any.whl", hash = "sha256:1d8214789a24de455a8b8bd8ae6fe3c6b69a5e3d64aa8a8e5d68e694bbcb285a"},
+    {file = "python_dotenv-1.2.2.tar.gz", hash = "sha256:2c371a91fbd7ba082c2c1dc1f8bf89ca22564a087c2c287cd9b662adde799cf3"},
 ]
 
 [package.extras]
diff --git a/pathology-api/pyproject.toml b/pathology-api/pyproject.toml
index 5ee0378..a282907 100644
--- a/pathology-api/pyproject.toml
+++ b/pathology-api/pyproject.toml
@@ -53,7 +53,7 @@ dev = [
     "schemathesis>=4.4.1",
     "types-requests (>=2.32.4.20250913,<3.0.0.0)",
     "types-pyyaml (>=6.0.12.20250915,<7.0.0.0)",
-    "python-dotenv (>=1.2.1,<2.0.0)",
+    "python-dotenv (>=1.2.2,<2.0.0)",
     "pytest-nhsd-apim (>=6.0.6,<7.0.0)",
 ]